Generator
Part:BBa_K325905:Design
Designed by: Will Handley and Emily Knott Group: iGEM10_Cambridge (2010-10-23)
Bacterial Lux reporter
CDEG pλ AB
(E. coli optimised)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3112
Illegal AgeI site found at 3327
Illegal AgeI site found at 3915
Illegal AgeI site found at 4898 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 5417
Design Notes
This part is a PoPS->light device that needs to be placed under a promoter and induced. It has been assembled from the parts LuxCD (BBa_K325902), LuxEG (BBa_K325903), a RiPS generator using plambda (BBa_K081006) and LuxAB (BBa_K216008) using the method of Gibson Assembly. Therefore no BioBrick scars have been created.
Source
a